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Image Search Results
Journal: Journal for Immunotherapy of Cancer
Article Title: KLRG1 marks tumor-infiltrating CD4 T cell subsets associated with tumor progression and immunotherapy response
doi: 10.1136/jitc-2023-006782
Figure Lengend Snippet: Expression of KLRG1 on tumor-infiltrating CD4 T cells over time in MC38 and NPK-C1. (A) Schematic summary of study design, models used, and analysis pipeline. (B) Dimensionality reduction (UMAP) and clustering (FlowSOM) of NPK-C1 (B) and MC38 (C) infiltrating immune cells, focusing on CD4 + Treg and Tconv populations. Cell-type subclustering, expression of FoxP3 and KLRG1 by heat map coloring, UMAP time courses, and frequencies of KLRG1 expression in each cell type. (D) Expression of phenotypic markers on all CD4 T cell sub-clusters across NPK-C1 and MC38 markers. Color scale is normalized to min/max of the cell type or all immune cells, as appropriate. Quantitation of KLRG1 expression in other immune cell types in the tumor (E) TDLN (F), NDLNs (G), and blood (H). Data were generated from one experiment of n=10–25 mice per/condition and time point. Statistical significance is denoted by **p<0.01, ***p<0.001, ****p<0.0001. NDLNs, non-draining lymph nodes; TDLN, tumor-draining lymph node.
Article Snippet: Flow cytometry data was analyzed in FlowJo V.10.8.1 (BD; Franklin Lakes, New Jersey, USA) and high dimensional plugin algorithms UMAP and
Techniques: Expressing, Quantitation Assay, Generated
Journal: Journal for Immunotherapy of Cancer
Article Title: KLRG1 marks tumor-infiltrating CD4 T cell subsets associated with tumor progression and immunotherapy response
doi: 10.1136/jitc-2023-006782
Figure Lengend Snippet: Association between Helios - KLRG1 + Treg frequency and tumor progression. (A) UMAP projection and FlowSOM clustering of NPK-C1 tumor Tregs, and cluster heatmap of normalized phenotypic marker expression. Pearson correlation of each cluster frequency versus tumor mass shown by heat map, and selected correlation dot plots in (B). (C) Schematic of NPK-C1 model with colors representing escaping tumors (>125 mg) versus those remaining in equilibrium (<80 mg) on D24. (D) UMAP projections of CD4 Treg metaclusters and pseudocolor representations of cells from escape tumors versus equilibrium tumors. (E) Violin plot of Helios - KLRG1 + Treg frequency in escape versus equilibrium tumors. (F) Schematic of MC38 model. (G) UMAP projections of CD4 Treg metaclusters and pseudocolor representations of cells from isotype versus anti-PD-1-treated tumors. (H) Violin plot of Helios - KLRG1 + Treg frequency in isotype versus anti-PD-1 tumors on day 10 and day 13. Data were generated from one experiment of n=10–25 mice per/condition and time point.
Article Snippet: Flow cytometry data was analyzed in FlowJo V.10.8.1 (BD; Franklin Lakes, New Jersey, USA) and high dimensional plugin algorithms UMAP and
Techniques: Marker, Expressing, Generated